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. Author manuscript; available in PMC: 2020 Nov 4.
Published in final edited form as: Mucosal Immunol. 2020 May 4;13(6):908–918. doi: 10.1038/s41385-020-0290-x

Figure 5. bTLT size and number are independent of microbial status and dependent on age-associated TNFα.

Figure 5.

(A) Representative H&E image of WT aged germ free (GF) bladders. (B) Number and size of bTLT found in WT specific pathogen free (SPF) and GF bladders. n=9–17/group. (B) Relative expression of Tnf and in young and aged bladders by RT-qPCR. n=10/group. (C) Representative H&E images of aged WT and TNFα−/− bladders. (C) Number and size of bTLT in aged WT and TNFα−/− bladders. Lines at median. *p<0.05, **p<0.01, Mann-Whitney U-test. Scale bars, 100 μm