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. 2020 Oct 19;10:17619. doi: 10.1038/s41598-020-71737-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Ultramicroscopy setup. Recording of the large tissue sample. (a) Ultramicroscopy setup: Sapphire laser unit for fluorescence excitation (not shown), a beam splitter cube (BSC), 45 degrees silver mirrors (M), two light sheet generator units (LSG), two linear stages (LS) that move the LSG units along the beam propagation axis (z) for superimposing the center of the light sheet in the center of the biological sample, a computer controlled stage for moving the sample through the light sheet vertically (VS), a quartz container (QC), filled with imaging media (DBE). The detecting unit contains × 2, × 4 or × 16 objective equipped with a modulator (MO) for compensating refractive index mismatch, a tube lens (TL) equipped with a band pass filters (BPF) wheel, and a CMOS Camera. (b,c) Cancer sample recording with × 2 magnification.