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. 2020 Oct 19;10:17619. doi: 10.1038/s41598-020-71737-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Post-processing of cleared specimen with standard histology methods. Comparison of obtained images. (a) Cleared specimen. (b) 4-dots labelling (red arrows) of the optical “area of interest”, found within the UM-recording. (c) Same sample, embedded in paraffin. (d,e) Optical sections of 3D-reconstruction of the specimen (see movie to Fig. 5d in Supplementary material), corresponding to (f,g) H&E-stained histological sections (× 2 magnification). (h) Whole-specimen cut with microtome and stained with H&E.