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. 2020 Oct 19;10:17619. doi: 10.1038/s41598-020-71737-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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3D imaging of low-grade invasive lobular adenocarcinoma of breast. (a–c) Representative 3D reconstructions of the sample recorded with × 2 and × 4 magnification, captured from different perspectives; sites of high cellular density corresponding to the invasive carcinoma are visualized as dark areas. (d) Optical section of 3D reconstruction of the sample recorded with × 4 magnification. (e) Corresponding physical section of the specimen (post-clearing), processed with standard histology, stained with H&E and recorded with × 5 magnification (see movies to Fig. 6 in Supplementary material).