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. Author manuscript; available in PMC: 2020 Dec 22.
Published in final edited form as: Nat Chem Biol. 2020 Jun 22;16(11):1189–1198. doi: 10.1038/s41589-020-0557-2

Extended Data Fig. 6. TP53 thermal stability.

Extended Data Fig. 6

(a) FLAG-UBR7, UBR7, and loading control GAPDH levels from 231MFP cells expressing FLAG-UBR7 after mock depletion or FLAG-UBR7 depletion as assessed by Western blotting. (b) TP53 thermal stability in cell lysate from 231MFP FLAG-UBR7-expressing cells treated with DMSO vehicle or asukamycin (50 μM, 1 h) after mock depletion or FLAG-UBR7 depletion. (c) Quantification of experiments described in (b). Gels in (a) are representative of n=3 biologically independent samples/group. Gels in (b) are representative of n=3 biologically independent samples/group which are quantified and shown as individual replicate values in (c). Statistical significance in (c) was calculated with two-tailed unpaired Student’s t-tests and are shown as *p<0.05 comparing UBR7-depleted asukamycin-treated groups to mock-depleted asukamycin-treated groups. Uncropped blots can be found in Source Data for Extended Figure 6. Source data for plots can be found in Source Data Tables for Extended Figure 6.