Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 19;10:17614. doi: 10.1038/s41598-020-74448-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Behavioural response of larvae expressing different light-sensitive ion channels in motor neurons (OK6-GAL4) to optogenetic stimulation with the Honor 8 smartphone display. (a) Motoneuronal expression of Chrimson::Venus obtained by antibody staining against the photoprotein (green). Phalloidin stained ventral longitudinal muscles (VLM) depicted in magenta. Scale bar: 10 µm. (b)–(f) Larval responses to illumination with different colours in temporal sequences of red (R), green (G), blue (B), and white (W) light with intermediate black periods (Supplementary Fig. S3). Data show the speed of the larvae normalised to the previous black period as obtained by tracking the head (see Methods). (b) Neuronal activation of OK6 > CsChrimson larvae. Stimulation period of 20 s with 60 s of rest (black period). (c) Neuronal activation using OK6 > ChR2^XXL. Stimulation period: 10 s; black period: 150 s. (d) Neuronal silencing using OK6 > GtACR1. Stimulation period: 20 s; black period: 60 s. (e) Neuronal silencing using OK6 > GtACR2. Stimulation period: 30 s; black period: 60 s. (f) Normalised speed of w¹¹¹⁸ control larvae. Stimulation period: 20 s; black period: 30 s. N: number of larvae; whiskers: s.e.m.; diamonds: mean. Significance calculated via one-sample two-tailed t-test: ns: not significant (p > 0.05), *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.