Figure 5.

Spatial control of larval movement by patterned illumination with a smartphone. (a) Guidance of free-behaving ppk > CsChrimson larvae. Images show representative frames from Video S4. The larva is enclosed within a ring of white light that moves at a speed of 0.17 mm/s in x-direction. The red track shows the larval trajectory. (b) Mean velocity < v_x > in x-direction and (c) mean speed < s > of 10 w¹¹¹⁸ control larvae and 10 ppk > CsChrimson larvae confined in the conditions described in (a). Whiskers: s.e.m.; diamonds: mean. Significance calculated via one-sample two-tailed t-test (in (b)) and two-sample two-tailed t-test (in (c)): ns: not significant (p > 0.05), ****p < 0.0001. (d) Confinement of 11 ppk > CsChrimson larvae inside a black maze surrounded by white light (Supplementary Video S6). Left: Image of larvae positioned inside the maze. Right: Tracks of 11 larvae over the course of 21 min 30 s. (e) Tracks of 10 w¹¹¹⁸ control larvae over the course of 21 min 30 s (Supplementary Video S7). (f) Histogram and Gaussian fit of the larval head positions along the red line (20 mm width) shown in (d). Dashed lines mark the 95% confidence interval.