Figure 4. Effects of CGRP monoclonal antibodies in repeated stress induced priming to SNP in mice.

Facial withdrawal thresholds were measured in male (A) and female (B) mice following acute stress (n = 10 males, n = 7 females). 15 days following the final day of stress, animals received either the anti-CGRP antibody ALD405 (n = 10 males, n = 8 females) or an isotype control (n = 9 males, n = 9 females). 24 hours following administration of antibody, all animals received 0.1 mg/kg SNP. In the acute phase, (†) denotes statistical significance between control mice and stressed mice that will receive ALD405 and SNP. (*) denotes statistical significance between control mice and stressed mice that will receive isotype control IgG. In the priming phase, (§) denotes statistical significance between stressed mice that received ALD405 and those that received the isotype control prior to SNP. Two-way ANOVA followed by Bonferroni multiple comparison analysis revealed significant differences in the priming phase between female stressed mice that received ALD405 and those that received the isotype. Statistical significance between these groups was observed in males at 48 hours following SNP. Data are represented as means ± SEM. ^§p<0.05,^§§p < 0.01, ^§§§p<0.001, ^{††††,}****p<0.0001. See Table 2 for additional results of analysis.