Fig. 6.

Blockade of GM-CSF decreases disease by suppressing migration and function of eosinophils. WT and Ifng^−/−Il17a^−/− mice on B10.RIII background were immunized with p161–180 and i.p. treated with anti-GM-CSF mAb (MP1–22E9.11) or isotype Ig (Rat IgG2a) from the effector phase (starting on day 7). (A) The number of eosinophils (SiglecF⁺CD11b⁺CD45⁺), neutrophils (Ly6G⁺Ly6C⁻CD11b⁺CD45⁺), monocytes (Ly6G⁻Ly6C⁺CD11b⁺CD45⁺) in eye-infiltrating cells was determined by FACS. (B) Representative FACS plots of eye-infiltrating eosinophils. (C) Eosinophil peroxidase (EPO) in the eye-supernatant and the serum were assessed by LEGENDPlex Representative data from 3 independent experiments, and each group contains at least 3 mice. Data shown as mean ± SEM. *p < 0.05, **p < 0.01, Student’s t-test.