Figure 10. CRMP2^K374A/K374A knock-in mice do not develop persistent mechanical allodynia in the spared nerve injury (SNI) model of neuropathic pain.

Paw withdrawal thresholds of age-matched and genotyped WT and CRMP2^K374A/K374A mice were measured at baseline and for five weeks following SNI. Post SNI, von Frey testing was confined to the sural nerve innervating region of the paw. Time course (A – females; C – males) and area under the curve (B – females; D – males) are shown. Area under the curve for paw withdrawal thresholds was derived using the trapezoid method. von Frey mechanical thresholds indicating that loss of CRMP2 SUMOylation prevented the development of mechanical allodynia after SNI in both male and female CRMP2^K374A/K374A mice. See statistical analysis described in Table 2. Error bars indicate mean ± SEM. The experiments were conducted by investigators blinded to the genotype.