Figure 4. Preventing CRMP2 SUMOylation decreases thermal sensitivity in males and females.

Latencies to respond to noxious heat (48˚C, 52˚C, or 55˚C) in the hot plate (A-F) or tail-flick (52˚C) (G, H) tests in female and male wildtype (WT) and CRMP2^K374A/K374A mice. Paw withdrawal latency was increased in heterozygous males at 48°C vs. wildtype males and increased at 52°C between wildtype and homozygotes males. There were no changes in latencies at the highest temperature. At 48°C, female CRMP2^K374A/K374A mice exhibited a higher latency to respond to the nociceptive stimulus compared to wildtype mice. There were no changes in latencies at 52°C or 55°C. There were no changes in the hot-plate test under any of the conditions; the test was stopped at the cutoff time of 10-s. See statistical analysis described in Table 2. Error bars indicate mean ± SEM.