Figure 8. PFOR inactivation depends upon the concentration of oxygen.

Exponentially growing WT cells (BT5482) in anoxic BHIS were washed, suspended in Tris buffer containing glucose and chloramphenicol, and incubated with the indicated percent of air saturation for 1 hr at 37 °C in tightly closed bottles. Cells were then harvested and their PFOR activities determined. The dotted line represents prior data (Lu et al., 2018) showing that the fumarase inactivation by superoxide parallels PFOR inactivation by oxygen. Error bars represent the standard error of the mean of six biological replicates.