Figure 5.
Macrophage depletion in obese mice led to increased p53−/− MECs with DNA damage. A. Representative comet assays from p53−/− MECs. Percent DNA in tail and olive moment were quantified from 50 cells/recipient (n=3/group; Kruskal-Wallis test with Dunn’s multiple comparison post-test). Bars represent geometric means. B. Percent of BrdU+ MECs (n=3–4/group). C. Percent of MECs labeled for γH2AX and BrdU (n=3–4/ group). D. Percent of MECs labeled for 8-OHdG (n=3–4/group). E. Tumor incidence and image of p53−/− spindle cell carcinomas that formed in aF4/80-treated HFD-fed mice (P=0.06, chi-squared test for trend). Magnification bar=50 μm.