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. Author manuscript; available in PMC: 2021 Apr 7.
Published in final edited form as: Nature. 2020 Oct 7;586(7829):424–428. doi: 10.1038/s41586-020-2812-9

Extended Data Table 6 |.

Immunohistochemical markers and applications.

Marker Antibody Antigen Retrieval Secondary reagents
Active caspase 3 Anti-Active Caspase 3 (Promega, Walldorf, Germany), 1:200, overnight n/a ABC Kit Vectastain Elite PK 6100, 30 min (Dako)
CD79a Mouse anti-CD79A (clone HM57) monoclonal, (LifeSpan BioSciences, Seattle, WA, USA), 1:50, overnight HIER, 10 mM Tris/1mM EDTA buffer pH 9.0, 20 min Dako EnVision+ System-HRP Labelled Polymer Anti-mouse, 30 min
CD3 Rabbit anti-CD3 polyclonal (Dako), 1:100, overnight HIER, 10 mM Tris/1mM EDTA buffer pH 9.0, 20 min Dako EnVision+ System- HRP Labelled Polymer Anti-rabbit, 30 min
Iba-1 Iba1 (Wako), 1:800, overnight HIER, Citrate buffer pH 6.0, for 20 min Dako EnVision+ System- HRP Labelled Polymer Anti-rabbit, 30 min

HIER: Heat-induced epitope retrieval; HRP: horse-radish peroxidase. n/a = not applicable.