Figure 7:

Behavioral analysis of zebrafish embryos injected with shh MO and exposed to ethanol during late gastrulation to early neurulation (8–10 hpf). Embryos were assessed for novel tank diving response at 60–75 days. As controls, embryos were either untreated (Control), exposed to ethanol alone or injected with shh MO or fgf8 mRNA alone. Altered tank diving response, indicative of increased risk-taking behavior, is only observed for fish exposed to both shh MO and ethanol. Overexpression of fgf8 mRNA was able to attenuate the risk-taking behavior. Each bar represents the mean distance from the tank floor for one minute (min 1–5). Error bars represent SEM. (**significantly different from control (post-hoc) across all time points analyzed, P<0.001). N≥ 15 per group.