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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Biochem Pharmacol. 2020 Jun 25;181:114124. doi: 10.1016/j.bcp.2020.114124

Figure 5:

Figure 5:

The comparison of Arg13 analogs tested against human α9α10 nAChRs, obtained by two-electrode voltage clamp. Each analog was flowed on at 10 nM and allowed to reach equilibrium. Each data plot represents the mean of at least 3 replicate samples accompanied with the standard error of the mean. The IC50 values of each RgIA analog compared to the native RgIA by ordinary one-way ANOVA followed by a Dunnett’s multiple comparison test, shown with the SEM. A designation of three asterisks (***) indicates a value of P<0.001, and four asterisks (****) indicates a value of P <0.0001. Non-natural amino acids were utilized to specifically test for electronegativity vs. aromaticity of the side chain moieties. Non-natural amino acids include L-tyrosine amide (Tyr#); L-phenylalanine amide (Phe#), L-4-carboxyl-phenylalanine amide (4-COOH-Phe#); 1-naphthalenealanine (1-Nal); 2-naphthalenealanine (2-Nal).