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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Brain Behav Immun. 2020 May 23;89:20–31. doi: 10.1016/j.bbi.2020.05.058

Figure 1.

Figure 1.

Experimental Design. Bone marrow was extracted from postnatal day 28 (P28) male BTBR or C57 mice and differentiated in culture for seven days (DIV 7) to form bone marrow derived macrophages (BMDM). BMDM from each mouse were re-seeded into three wells and treated with either media, one dose of LPS for 24hrs (LPSx1) or given a 4hrs LPS pretreatment followed by a 24hrs LPS treatment (LPSx2). All LPS treatments were at 100ng/ml of culture media. Following treatment, cells were collected for RNA expression analysis (Fluidigm array) or chromatin accessibility (ATAC-sequencing).