Table 2. Nrf2-target gene expression changes in CoPP-treated and B-RafV600E-transformed Hs936T cells.
Statistically significant genes were identified using the DESeq R Bioconductor package.
| Hs936T-B-RafV600E | CoPP Treatment | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene | ID | Fold change | P-value | Qvalue | Significant | Fold change | P-value | Qvalue | Significant | |
| ARE Regulation | ||||||||||
| NFE2L2 | NM_006164 | 0.65 | 4.18E-11 | 1.40E-10 | yes | 1.14 | 2.21E-03 | 8.38E-03 | yes | |
| BACH1 | NM_206866 | 1.45 | 2.88E-02 | 4.36E-02 | yes | 1.02 | 7.23E-01 | 8.22E-01 | no | |
| MAFK | NM_002360 | 1.17 | 2.24E-01 | 2.83E-01 | no | 0.82 | 3.37E-03 | 1.21E-02 | yes | |
| MAFF | NM_001161572 | 0.37 | 2.80E-21 | 1.57E-20 | yes | 1.52 | 2.36E-15 | 7.46E-14 | yes | |
| MAFG | NM_032711 | 0.31 | 5.30E-60 | 1.00E-58 | yes | 1.05 | 3.12E-01 | 4.65E-01 | no | |
| Heme Degradation | ||||||||||
| HMOX1 | NM_002133 | 0.07 | 6.01E-185 | 8.35E-183 | yes | 14.20 | 1.10E-183 | 1.35E-179 | yes | |
| FTH1 | NM_002032 | 0.35 | 4.79E-44 | 6.09E-43 | yes | 1.58 | 9.68E-20 | 5.24E-18 | yes | |
| FTL | NM_000146 | 0.15 | 1.73E-108 | 8.65E-107 | yes | 0.98 | 6.95E-01 | 8.01E-01 | no | |
| ME1 | NM_002395 | 162.97 | 4.02E-69 | 9.28E-68 | yes | 1.23 | 2.23E-01 | NA | no | |
| Detoxifying Enzymes | ||||||||||
| NQO1 | NM_000903 | 0.24 | 1.29E-74 | 3.33E-73 | yes | 2.32 | 9.50E-82 | 1.30E-78 | yes | |
| NQO2 | NM_000904 | 0.34 | 2.44E-34 | 2.29E-33 | yes | 1.37 | 2.34E-08 | 2.75E-07 | yes | |
| GSTM1 | NM_000561 | 59.24 | 4.30E-22 | 2.51E-21 | yes | 1.02 | 8.52E-01 | NA | no | |
| Glutathione Metabolism | ||||||||||
| GCLC | NM_001498 | 0.75 | 6.26E-03 | 1.05E-02 | yes | 0.99 | 8.64E-01 | 9.16E-01 | no | |
| GCLM | NM_002061 | 0.56 | 6.50E-05 | 1.36E-04 | yes | 2.23 | 1.68E-32 | 2.51E-30 | yes | |
| SLC7A11 | NM_014331 | 0.14 | 2.25E-80 | 6.60E-79 | yes | 1.83 | 8.28E-26 | 7.62E-24 | yes | |
| G6PD | NM_000402 | 0.62 | 5.02E-09 | 1.47E-08 | yes | 1.77 | 1.28E-39 | 3.14E-37 | yes | |
| Cell Cycle and Apoptosis | ||||||||||
| SQSTM1 | NM_003900 | 0.10 | 1.44E-127 | 1.01E-125 | yes | 0.93 | 6.75E-02 | 1.45E-01 | no | |
| TFE3 | NM_006521 | 0.73 | 1.90E-03 | 3.40E-03 | yes | 0.94 | 2.18E-01 | 3.59E-01 | no | |
| BCL2L11 | NM_138621 | 0.45 | 3.06E-09 | 9.12E-09 | yes | 0.82 | 7.13E-03 | 2.28E-02 | yes | |
| Additional Antioxidants | ||||||||||
| TXNRD1 | NM_001093771 | 0.33 | 5.02E-69 | 1.15E-67 | yes | 1.33 | 7.32E-11 | 1.25E-09 | yes | |
| SOD3 | NM_003102 | 402.13 | 4.35E-30 | 3.42E-29 | yes | 0.95 | 3.47E-01 | NA | no | |
| Growth Factors | ||||||||||
| TGFB2 | NM_001135599 | 4.61 | 2.11E-03 | 3.74E-03 | yes | 0.98 | 8.72E-01 | NA | no | |
| TGFB1 | NM_000660 | 7.44 | 5.80E-110 | 3.04E-108 | yes | 1.59 | 2.31E-07 | 2.28E-06 | yes | |
| FGF13 | NM_004114 | 1.96 | 2.11E-11 | 7.23E-11 | yes | 1.82 | 5.57E-16 | 1.91E-14 | yes | |
| Proteosome | ||||||||||
| PSMB3 | NM_002795 | 1.43 | 5.84E-05 | 1.23E-04 | yes | 0.89 | 6.29E-02 | 1.37E-01 | no | |
| PSMA4 | NM_002789 | 2.53 | 2.03E-30 | 1.62E-29 | yes | 1.14 | 3.62E-02 | 8.79E-02 | yes | |
| PSMA1 | NM_148976 | 1.66 | 5.80E-11 | 1.93E-10 | yes | 1.17 | 6.95E-03 | 2.23E-02 | yes | |
| PSMB6 | NM_002798 | 1.84 | 7.85E-10 | 2.43E-09 | yes | 0.86 | 3.61E-02 | 8.77E-02 | yes | |