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. Author manuscript; available in PMC: 2020 Oct 20.
Published in final edited form as: Biochemistry. 2020 Sep 30;59(40):3802–3812. doi: 10.1021/acs.biochem.0c00690

Figure 4.

Figure 4.

Exemplary results of a competitive ELISA with wt and alanine-substituted cdMMP-14. Immobilized cdMMP-14 wt was incubated with Fabs (3A2, 3D9, and DX-2400 at 10 nM; 3E2, 2B5, and 3E9 at 20 nM) in the presence of 1 nM to 3 μM cdMMP-14 mutants. Captured Fabs were detected with anti-Fab-HRP for signal development. Four scenarios were observed. (A) Binding was abolished with the cdMMP-14 mutant. (B) The binding affinity was weakened with the mutant compared to cdMMP-14 wt. (C) The binding affinity was unchanged. (D) The binding affinity was enhanced with the cdMMP-14 mutant.