KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-human CD3 APC/Cy7 (clone OKT3) | BioLegend | Cat#317341 |
| Anti-human CD8 V450 (clone RPA-T8) | BD Biosciences | Cat#560347 |
| Anti-human CD14 BV785 (clone M5E2) | BioLegend | Cat#301839 |
| Anti-human CD20 Alexa Fluor 700/PE (clone 2H7) | BioLegend | Cat#302322 |
| Anti-human IgM PE/Cy5 (clone G20-127) | BD Biosciences | Cat#551079 |
| Anti-human IgG APC (clone G18-145) | BD Biosciences | Cat#550931 |
| Anti–human IgG, HRP conjugated | Bethyl Laboratories | Cat#A80-119P |
| Anti–human IgG, Sulfo-tag | Meso Scale Discovery | Cat#R32AJ-1 |
| Anti-human IgG-Alexa Fluor® 647 | Thermo Fisher Scientific | Cat#A-21445 |
| Anti-PfCSP antibody, 3SP2-FITC | (Verhave et al., 1988) | N/A |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant PfCSP constructs with truncated repeat regions and minor repeat mutations | This Paper | GenBank Accession Numbers MT891160 - MT891178 |
| Fluorescein Isothiocyanate Streptavidin | BD Biosciences | Cat#554060 |
| Brilliant™ Violet 605 Streptavidin | BD Biosciences | Cat#563260 |
| Dextran, Rhodamine B | Thermo Fisher Scientific | Cat#D1824 |
| Bir A | Avidity | Cat#BirA500 |
| RNaseOUT™ Recombinant Ribonuclease Inhibitor | Thermo Fisher Scientific | Cat#10777019 |
| IgePAL | Millipore Sigma | Cat#I8896 |
| Alexa Fluor™ 405 NHS Ester (Succinimidyl Ester) | Thermo Fisher Scientific | Cat#A30000 |
| 1-Step™ Ultra TMB-ELISA | Thermo Fisher Scientific | Cat#34029 |
| Accudenz density gradient | Accurate Chemical | Cat#AN7050 |
| E-64 | Millipore Sigma | Cat#E3132 |
| D-Luciferin | Perkin Elmer | Cat#1227991 |
| eBioscience™ Fixable Viability Dye eFluor™ 506/780 | Thermo Fisher Scientific | Cat#65-2860-40 |
| Avertin | Alfa Aesar | Cat#A18706 |
| Critical Commercial Assays | ||
| SuperScript™ First-Strand Synthesis System for RT-PCR | Thermo Fisher Scientific | Cat#11904018 |
| LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit | Thermo Fisher Scientific | Cat#L34966 |
| Expi293™ Expression System Kit | Thermo Fisher Scientific | Cat#A14635 |
| FreeStyle™ 293 Expression System | Thermo Fisher Scientific | Cat#K900001 |
| rProtein A Sepharose® Fast Flow | Millipore Sigma | Cat#GE17-1279-03 |
| SAIVI™ Alexa Fluor™ 647 Antibody/Protein Labeling Kit | Thermo Fisher Scientific | Cat#S30044 |
| Deposited Data | ||
| Human PfCSP mAb heavy and light chain sequences | This Paper | GenBank Accession Numbers MT811859 - MT811914 |
| Experimental Models: Cell Lines | ||
| Human: HC-04 hepatoma cells | (Sattabongkot et al., 2006) | N/A |
| Human: Expi293 cells | Thermo Fisher Scientific | Cat#A14527 |
| Human: 293F cells | Thermo Fisher Scientific | Cat#R79007 |
| Experimental Models: Organisms/Strains | ||
| Mouse: B6(Cg)-Tyrc-2J/J albino | The Jackson Laboratory | Cat #000058 |
| Mouse: C57BL/6 | Charles River Laboratories | Cat#027 |
| Mouse: Swiss Webster | Charles River Laboratories | Cat#024 |
| Mouse: FRG huHep | Yecuris Corp | Cat#10-0006 |
| Sporozoite: P. berghei sporozoite expressing PfCSP, GFP, and luciferase | (Flores-Garcia et al., 2019) | N/A |
| Sporozoite: P. berghei sporozoite expressing PfCSP, and GFP | This Paper | N/A |
| Sporozoite: P. falciparum sporozoite, strain NF54 | (Delemarre-van de Waal and de Waal, 1981) | N/A |
| Oligonucleotides | ||
| PCR primers for amplification of antibody heavy, kappa, and lambda genes | This paper | GenBank Accession Numbers MT811859 - MT811914 |
| Recombinant DNA | ||
| Plasmodium falciparum circumsporozoite protein (clone 3D7) | PlasmoDB | PF3D7_0304600.1 |
| pVRC8400 huIgG1 | Genscript | N/A |
| pVRC8400 huIgK | Genscript | N/A |
| SBShuLambda | Genscript | N/A |
| Software and Algorithms | ||
| FlowJo 9 software | FlowJo | https://www.flowjo.com; RRID:SCR_008520 |
| The International Immunogenetics Information System | IMGT | imgt.org; RRID:SCR_006931 |
| GraphPad Prism 7 software | GraphPad Software | https://www.graphpad.com; RRID:SCR_002798 |
| RStudio | RStudio | https://rstudio.com/; RRID:SCR_000432 |
| Octet Software, version 7.0 | FortéBio | http://www.fortebio.com; |
| Living Image Software, version 4.5 | Perkin Elmer | http://perkinelmer.com; RRID:SCR_014247 |
| Imaris | Bitplane | https://imaris.oxinst.com/; RRID:SCR_007370 |
| Rosetta | Rosetta Commons | https://www.rosettacommons.org/software; |
| Geneious Prime | Geneious | http://www.geneious.com; RRID:SCR_010519 |
| lme4 | R package | https://cran.r-project.org/web/packages/lme4/index.html; RRID:SCR_015654 |
| arm | R package | https://cran.r-project.org/web/packages/arm/index.html |
| tidyverse | R package | https://cran.r-project.org/web/packages/tidyverse/index.html |
| Other | ||
| BD LSRFortessa II | BD Biosciences | N/A |
| BD FACSAria II cell sorter | BD Biosciences | N/A |
| SECTOR Image 2400 | Meso Scale Discovery | N/A |
| U-PLEX Assay Platform | Meso Scale Discovery | N/A |
| Octet HTX | FortéBio | N/A |
| VP-ITC microcalorimeter | Malvern Panalytical | N/A |
| Gallios Flow Cytometer | Beckman Coulter | N/A |
| IVIS® Spectrum In Vivo Imaging System | Perkin Elmer | N/A |
| Fluoview FVMPE-RS Multiphoton Microscope System | Olympus | N/A |