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. Author manuscript; available in PMC: 2021 Oct 14.
Published in final edited form as: Neuron. 2020 Aug 26;108(1):193–208.e9. doi: 10.1016/j.neuron.2020.07.023

Figure 2. n-Tr20 Is Primarily Expressed in Neurons.

Figure 2.

(A) Northern blot of the expression of n-Tr20 and other tRNAArgUCU genes. 5S rRNA was used as a loading control.

(B–E) In situ hybridization of n-Tr20 (red) and immunohistochemistry for cell-type markers (brown) in the hippocampus (B and C), cortex (D), and cerebellum (E). Cortical layers are indicated by Roman numerals. DG, dentate gyrus, ML, molecular layer; PCL, Purkinje cell layer; GCL, granule cell layer. Scale bars represent 100 μm (D), 50 μm (B), and 25 μm (B and D [higher magnification], C, and E).