| Antibodies |
|
|
| SH-PTP2(B-1) |
Santa Cruz |
Cat# sc-7834; RRID:AB_628252 |
| Phospho-p44/p42 MAPK(Erk1/2)(Thr202/Tyr204) |
Cell Signaling Technology |
Cat# 4370S; RRID:AB_2315112 |
| p44/p42 MAPK(Erk1/2) |
Cell Signaling Technology |
Cat# 9102S; RRID:AB_330744 |
| Phospho-MEK1/2(Ser217/221)(41G9) |
Cell Signaling Technology |
Cat# 9154; RRID:AB_2138017 |
| MEK1/2 |
Cell Signaling Technology |
Cat# 9122; RRID:AB_823567 |
| SHP2 |
Cell Signaling Technology |
Cat# 3397T; RRID:AB_2174959 |
| HA |
Cell Signaling Technology |
Cat# 2367S; RRID:AB_10691311 |
| FLAG |
Sigma-Aldrich |
Cat# F1804; RRID:AB_262044 |
| GAPDH |
Cell Signaling Technology |
Cat# 2118S; RRID:AB_561053 |
| α-tubulin |
Abcam |
Cat# ab52866; RRID:AB_869989 |
| Bacterial and Virus Strains |
|
Escherichia coli BL21(DE3) |
TIANGEN |
Cat# CB105–01 |
|
Escherichia coli Stbl3 |
Exinbio |
Cat# CC104–01 |
|
Escherichia coli DH5α |
TIANGEN |
Cat# CB101–01 |
| Biological Samples |
| Human oral mucosal epithelial cells |
This study |
N/A |
| Chemicals, Peptides, and Recombinant Proteins |
| 2P-IRS-1 peptide |
QiangYao |
N/A |
| 6,8-difluoro-4-methylumbelliferyl phosphate(DiFMUP) |
Invitrogen |
Cat# D22065
|
| p-Nitrophenyl Phosphate(PNPP) |
NEB |
Cat# P0757S |
| Doxycycline hyclate |
Yeasen |
Cat# 60204ES08 |
| G418 Sulfate |
Yeasen |
Cat# 60220ES03 |
| Puromycin Dihydrochloride |
ThermoFisher Scientific |
Cat# A1113802 |
| SHP099 |
This study |
N/A |
| ET070 |
This study |
N/A |
| Recombinant SHP2(WT) protein |
This study |
N/A |
| Recombinant SHP2(D61G) protein |
This study |
N/A |
| Recombinant SHP2(E76A) protein |
This study |
N/A |
| Recombinant SHP2(E76K) protein |
This study |
N/A |
| Recombinant SHP2(Y279C) protein |
This study |
N/A |
| Recombinant SHP2(R498L) protein |
This study |
N/A |
| Recombinant SHP2(Q506P) protein |
This study |
N/A |
| Recombinant SHP2(G464A) protein |
This study |
N/A |
| Recombinant SHP2(PTP) protein |
This study |
N/A |
| Recombinant SHP2(1–528) protein |
This study |
N/A |
| Recombinant SHP2(C+N-SH2) protein |
This study |
N/A |
| Recombinant SHP1 protein |
This study |
N/A |
| Recombinant PTP1B protein |
This study |
N/A |
| Recombinant FAP1 protein |
This study |
N/A |
| Recombinant CD45 protein |
This study |
N/A |
| Recombinant VHR protein |
This study |
N/A |
| Recombinant LMWPTP protein |
This study |
N/A |
| Recombinant MKP3 protein |
This study |
N/A |
| Recombinant SHP2(WT) –mEGFP protein |
This study |
N/A |
| Recombinant SHP2(E76A) –mEGFP protein |
This study |
N/A |
| Recombinant SHP2(E76K) –mEGFP protein |
This study |
N/A |
| Recombinant SHP2(Y279C) –mEGFP protein |
This study |
N/A |
| Recombinant SHP2(R498L) –mEGFP protein |
This study |
N/A |
| Recombinant SHP2(G464A) –mEGFP protein |
This study |
N/A |
| Recombinant SHP2(Q506P) –mEGFP protein |
This study |
N/A |
| Recombinant SHP2(T468M) –mEGFP protein |
This study |
N/A |
| Recombinant SHP2(E76A) –mScarlet protein |
This study |
N/A |
| Recombinant SHP2(E76K) –mScarlet protein |
This study |
N/A |
| Recombinant SHP2(Y279C) –mScarlet protein |
This study |
N/A |
| Recombinant SHP2(R498L) –mScarlet protein |
This study |
N/A |
| Recombinant PTPE523K/D437K protein |
This study |
N/A |
| Recombinant PTPR278E/K280E protein |
This study |
N/A |
| Recombinant PTPR265E protein |
This study |
N/A |
| Recombinant PTPE249K/E250K protein |
This study |
N/A |
| Recombinant PTPR362E/K364E protein |
This study |
N/A |
| Recombinant PTPE447K/E481K protein |
This study |
N/A |
| Recombinant PTPE441K/D437K protein |
This study |
N/A |
| Recombinant PTPE447K/D451K protein |
This study |
N/A |
| Recombinant PTPD431K/D395K protein |
This study |
N/A |
| Recombinant PTPD373KE390K protein |
This study |
N/A |
| Recombinant PTPD294K/E299K protein |
This study |
N/A |
| Recombinant PTPD296K/E299K protein |
This study |
N/A |
| Recombinant PTPD373A/E374A protein |
This study |
N/A |
| Recombinant PTPD303K/D296K protein |
This study |
N/A |
| Recombinant PTPK358A/R421A protein |
This study |
N/A |
| Recombinant PTPK266E/R270E protein |
This study |
N/A |
| Recombinant PTPK274E/K276E protein |
This study |
N/A |
| Recombinant PTPE523K/D437K –mEGFP protein |
This study |
N/A |
| Recombinant PTPR278E/K280E –mEGFP protein |
This study |
N/A |
| Recombinant PTPR265E –mEGFP protein |
This study |
N/A |
| Recombinant PTPE249K/E250K –mEGFP protein |
This study |
N/A |
| Recombinant PTPR362E/K364E –mEGFP protein |
This study |
N/A |
| Critical Commercial Assays |
| CellTiter-Glo reagent |
Promega |
Cat# G7570 |
| Deposited Data |
|
|
| Crystal structure of SHP2 |
Yu et al., 2013 |
PDB: 4DGP |
| Crystal structure of catalytic domain of human SHP2 |
Grosskopf et al. 2015 |
PDB: 3ZM0 |
| Experimental Models: Cell Lines |
|
|
| Human: KYSE520 |
Cobioer, Nanjing |
Cat# CBP60658 |
| Human: CCF-STTG1 |
Cobioer, Nanjing |
Cat# CBP60580 |
| Human: H661 |
Cell Bank of SIBCB |
Cat# TCHu121 |
| Human: NCI-H1299 |
Cell Bank of SIBCB |
Cat# SCSP-589 |
| Human: A549 |
Cell Bank of SIBCB |
Cat# SCSP-503 |
| Human: MV4;11 |
Cobioer, Nanjing |
Cat# CBP60522 |
| Human: SF268 |
Zhang Wei-Min lab |
N/A |
| Human: HEK293FT |
Wang Wenyuan lab |
N/A |
| Human: HEK293T |
Hu Junhao lab |
N/A |
| Human: HUVEC |
Promocell |
C-12203 |
| Mouse: MSC (bone marrow) |
Shi Yufang lab |
N/A |
| Mouse: E76K/+ MSC (bone marrow) |
Wang Siying lab |
N/A |
| Mouse: MEF |
Wang Siying lab |
N/A |
| Mouse: D61G/+ MEF |
Wang Siying lab |
N/A |
| Oligonucleotides |
|
|
| SHP2 gRNA for Knock out 5’-GTTACTGACCTTTCAGAGGT-3’ |
This study |
N/A |
| SHP2 gRNA for Knock in 5’- AGATGAGAAAACCTGCCAAAACT-3’ |
This study |
N/A |
| Primer for PCR amplification of Knock in fragment: Forward: 5’ -TGACATCGCGGAGATGGTTT- 3’ |
This study |
N/A |
| Primer for PCR amplification of Knock in fragment: Reverse: 5’-GCTGAACTTGTGGCCGTTTA- 3’ |
This study |
N/A |
| Forward primer for Sanger sequencing: 5’-GAGAGAAGACAGTGCTAGAGTCTATG-3’ |
This study |
N/A |
| Reverse primer for Sanger sequencing: 5’-CATCTCCATTCTTCTCTTTTAATTGC-3’ |
This study |
N/A |
| Recombinant DNA |
| pET28a-SHP2(WT) |
This study |
N/A |
| pET28a-SHP2(PTP) |
This study |
N/A |
| pET28a-SHP2(1–528) |
This study |
N/A |
| pET28a-SHP2(C+N-SH2) |
This study |
N/A |
| pET28a-SHP2(E76A) |
This study |
N/A |
| pET28a-SHP2(E76K) |
This study |
N/A |
| pET28a-SHP2(Y279C) |
This study |
N/A |
| pET28a-SHP2(R498L) |
This study |
N/A |
| pET28a-SHP2(G464A) |
This study |
N/A |
| pET28a-SHP2(Q506P) |
This study |
N/A |
| pET28a-SHP2(T468M) |
This study |
N/A |
| pET28a-SHP1(243–541) |
This study |
N/A |
| pET28a-PTP1B(1–298) |
This study |
N/A |
| pET28a-FAP1(2163–2477) |
This study |
N/A |
| pET28a-CD45(624–1233) |
This study |
N/A |
| pET28a-VHR(2–185) |
This study |
N/A |
| pET28a-LMWPTP(2–158) |
This study |
N/A |
| pET28a-MKP3(206–381) |
This study |
N/A |
| pET28a-SHP2(WT) -mEGFP |
This study |
N/A |
| pET28a-SHP2(E76A) -mEGFP |
This study |
N/A |
| pET28a-SHP2(E76K) -mEGFP |
This study |
N/A |
| pET28a-SHP2(Y279C) -mEGFP |
This study |
N/A |
| pET28a-SHP2(R498L) -mEGFP |
This study |
N/A |
| pET28a-SHP2(G464A) -mEGFP |
This study |
N/A |
| pET28a-SHP2(Q506P) -mEGFP |
This study |
N/A |
| pET28a-SHP2(T468M) -mEGFP |
This study |
N/A |
| pET28a-SHP2(E76A) -mScarlet |
This study |
N/A |
| pET28a-SHP2(E76K) -mScarlet |
This study |
N/A |
| pET28a-SHP2(Y279C) -mScarlet |
This study |
N/A |
| pET28a-SHP2(R498L) -mScarlet |
This study |
N/A |
| pET28a- PTPE523K/D437K
|
This study |
N/A |
| pET28a- PTPR278E/K280E
|
This study |
N/A |
| pET28a- PTPR265E
|
This study |
N/A |
| pET28a- PTPE249K/E250K
|
This study |
N/A |
| pET28a- PTPR362E/K364E
|
This study |
N/A |
| pET28a- PTPE447K/E481K
|
This study |
N/A |
| pET28a- PTPE441K/D437K
|
This study |
N/A |
| pET28a- PTPE447K/D451K
|
This study |
N/A |
| pET28a- PTPD431K/D395K
|
This study |
N/A |
| pET28a- PTPD373KE390K
|
This study |
N/A |
| pET28a- PTPD294K/E299K
|
This study |
N/A |
| pET28a- PTPD296K/E299K
|
This study |
N/A |
| pET28a- PTPD373A/E374A
|
This study |
N/A |
| pET28a- PTPD303K/D296K
|
This study |
N/A |
| pET28a- PTPK358A/R421A
|
This study |
N/A |
| pET28a- PTPK266E/R270E
|
This study |
N/A |
| pET28a- PTPK274E/K276E
|
This study |
N/A |
| pET28a- PTPE523K/D437K -mEGFP |
This study |
N/A |
| pET28a- PTPR278E/K280E -mEGFP |
This study |
N/A |
| pET28a- PTPR265E -mEGFP |
This study |
N/A |
| pET28a- PTPE249K/E250K -mEGFP |
This study |
N/A |
| pET28a- PTPR362E/K364E -mEGFP |
This study |
N/A |
| pMSCV-HA-SHP2(WT) |
This study |
N/A |
| pMSCV-FLAG-SHP2(WT) |
This study |
N/A |
| pMSCV-HA-SHP2(E76A) |
This study |
N/A |
| pMSCV-HA-SHP2(E76K) |
This study |
N/A |
| pMSCV-HA-SHP2(Y279C) |
This study |
N/A |
| pMSCV-HA-SHP2(R498L) |
This study |
N/A |
| pMSCV-HA-SHP2(D61G) |
This study |
N/A |
| pMSCV-HA-SHP2(WT)-mEGFP |
This study |
N/A |
| pMSCV-HA-SHP2(D61G)-mEGFP |
This study |
N/A |
| pMSCV-HA-SHP2(E76A)-mEGFP |
This study |
N/A |
| pMSCV-HA-SHP2(E76K)-mEGFP |
This study |
N/A |
| pMSCV-HA-SHP2(Y279C)-mEGFP |
This study |
N/A |
| pMSCV-HA-SHP2(R498L)-mEGFP |
This study |
N/A |
| pMSCV-HA-SHP2(WT)-mScarlet |
This study |
N/A |
| pMSCV-HA-SHP2(D61G)-mScarlet |
This study |
N/A |
| pMSCV-HA-SHP2(E76A)-mScarlet |
This study |
N/A |
| pMSCV-HA-SHP2(E76K)-mScarlet |
This study |
N/A |
| pMSCV-HA-SHP2(Y279C)-mScarlet |
This study |
N/A |
| pMSCV-HA-SHP2(R498L)-mScarlet |
This study |
N/A |
| pMSCV-HA-SHP2E76A/R362E/K364E-mEGFP |
This study |
N/A |
| pMSCV-HA-SHP2E76K/R362E/K364E-mEGFP |
This study |
N/A |
| pMSCV-HA-SHP2Y279C/R362E/K364E-mEGFP |
This study |
N/A |
| pMSCV-HA-SHP2R498L/R362E/K364E-mEGFP |
This study |
N/A |
| pInducer20-HA-SHP2Y279C-mEGFP |
This study |
N/A |
| pcDNA3.1-hAsCpf1 |
Zetsche et al.,2015 |
Addgene plasmid # 69982 |
| pUC57-U6-gRNA |
This study |
N/A |
| pUC57-PTPN11-HR-donor |
This study |
N/A |
| Software and Algorithms |
| LAS X |
Leica |
https://www.leicamicrosystems.com/products/microscopesoftware/details/product/leica-las-x-ls/ |
| PyMOL |
PyMOL |
https://pymol.sourceforge.net/ |
| Adobe Illustrator |
Adobe |
https://www.adobe.com/products/illustrator.html |
| ImageLab software |
Bio-Rad |
https://www.biorad.com |
| Fiji |
NIH |
https://imagej.nih.gov/ij/ |
| Graphpad Prism version 6.0 |
GraphPad Software |
https://www.graphpad.com |
