Table 2.

Distribution of hemoglobin variants and their RT

S. no.	Hb variant	No.	HbF (%) TOSOH G11	HbA2 (%) TOSOH G11	Other 1 (%)	Other 2 (%)	HbF (%) Bio-Rad (RT 1.08–1.17 min)
1	β thalassemia homozygous	3	86.5 ± 1.3	4.09 ± 0.68			87.43 ± 5.27
2	β thalassemia trait	25	0.9 ± 0.7	5.2 ± 0.74			1.09 ± 0.9
3	HbE β thalassemia	5	19.1 ± 8.5	8.2 ± 2.2	51.0 ± 3.4 HbE (RT 2.67 min)		17.34 ± 7.8
4	HbE heterozygous	20	0.8 ± 0.3	3.9 ± 0.4	22.4 ± 1.5 HbE (RT 2.67 min)		0.83 ± 0.5
5	HbE homozygous	8	2.7 ± 1.2	7 ± 0.79	71.4 ± 4.1 HbE (RT 2.67 min)		3.08 ± 1.49
6	HbD Punjab Heterozygous	11	0.7 ± 0.8	2.7 ± 0.49	32.15 ± 4.3 HbD (RT 2.82 –2.84 min)		0.918 ± 1.01
7	HbD Punjab homozygous	2	1.3 ± 1.4	5.4 ± 2.12	77.4 ± 0.6 HbD (RT 2.82 –2.84 min)		1.85 ± 1.9
8	HbDS Compound heterozygous	2	37.1 ± 0	1.6 ± 0	33.9 ± 0 HbD (RT 2.82 –2.84 min)	13.7 HbS (RT 3.11–3.16 min)	40.2
9	HbS heterozygous	12	1.02 ± 1.002	2.93 ± 0.67	26.8 ± 4.18 HbS (RT 3.11–3.16 min)		1.208 ± 1.18
10	HbS homozygous	8	18.48 ± 5.12	3.3 ± 1.4	61.6 ± 5.6 HbS (RT 3.11–3.16 min)		17.3 ± 4.6
11	HbSβ thalassemia	1	10.9	7.7	67.2 HbS (RT 3.11–3.16 min)		10.2
12	HbQ	2	0.37 ± 0.4	1.43 ± 0.09	12.0 ± 0.9 HbC window (RT 3.78 min)		0.3 ± 0.14
13	HbQ India with α	1	1.7	3.8	22.3 P07	12.7 P012	1.3
14	HbD Iran Heterozygous	4	0.67 ± 0.15	1.45 ± 0.48	35.35 ± 1.8 HbE (2.69 min)		0.425 ± 0.26
15	HbH	1	1.2	0.9	7.1 P00 (RT 0.13 min)		0.8
16	Unknown α variant	1	1.1	1.9	15.7 P04 (RT 1.27 min)		0.8
17	Hb Lepore trait	1	1.4	2.2	7.6 P08 (RT 2.67 min)		2.1
18	Normal	100	0.41 ± 0.56	2.53 ± 0.3			0.45 ± 0.59

S. no.	HbA2 (%) Bio-Rad (RT 3.55–3.66 min)	Other 1 (%)	Other 2 (%)	HbA2 (%) CZE	HbF (%) CZE	Others (%) CZE
1	3.7 ± 0.7
2	4.8 ± 0.6
3	69.8 ± 5.25			5.1 ± 1.6	21.4 ± 18.2	57.3 ± 2.19 HbE zone
4	26.7 ± 1.9			3.62 ± 0.37	0.9 ± 0.2	24.1 ± 1.14 HbE zone
5	84.6 ± 1.8			4.45 ± 0.8	13.7 ± 1.2	89.3 ± 3.16 HbE zone
6	1.6 ± 0.3	32.4 ± 3.7 HbD (RT 4.02–4.06 min)
7	2.3 ± 1.6	85.4 ± 1.4 HbD (RT 4.02–4.06 min)
8	1.6	35.3 ± 0 HbD (RT 4.02–4.06 min)	12.5 HbS (RT 4.33–4.37 min)
9	3.02 ± 0.73	30.98 ± 5.01 HbS (RT 4.33–4.37 min)
10	2.9 ± 1.04	73.07 ± 6.8 HbS (RT 4.33–4.37 min)
11	5.5	74.8 HbS (RT 4.33–4.37 min)
12	1.15 ± 0.07	15 Unknown (RT 4.7 min)		1.55 ± 0.5		17.05 ± 0.4 HbD zone
13	3.8	26.9 P03 (RT 1.77 min)	9.1 Unknown (RT 4.67 min)	3.3	0.2, 27.9 of Unknown 3 in Zone 12	0.2 HbE zone; 10.6 HbD zone
14	40.1 ± 1.87 (RT 3.53 min)			2.15		43.2 ± 0.04 HbD zone
15	1.2	RT < 1 min
16	1.9	23.7 P03 (RT 3.64 min)
17	8.9 (RT 3.46 min)			2		7.6 HbD zone
18	2.65 ± 0.27