Table 2.
Combinational effects of eugenol and antimicrobial drugs on pre-formed C. albicans and S. mutans MTCC497 single and mixed biofilms
| Test strains | Test combinations (concentration) | |||
|---|---|---|---|---|
| 1. CAJ-01 | EUG/FLZ (µg/mL) | EUG/AMB (µg/mL) | ||
| EUG | FLZ | EUG | AMB | |
| Alone MIC | 400 | 512 | 400 | 256 |
| Combination MIC | 50 | 64 | 100 | 128 |
| FICI | 0.250 | 0.625 | ||
| Type of interaction | Synergy | Indifferent | ||
| 2. CAJ-12 | EUG/FLZ (µg/mL) | EUG/AMB (µg/mL) | ||
| EUG | FLZ | EUG | AMB | |
| Alone MIC | 200 | 1024 | 200 | 32 |
| Combination MIC | 6.25 | 128 | 25 | 16 |
| FICI | 0.156 | 0.625 | ||
| Type of interaction | Synergy | Indifferent | ||
| 3. S. mutans MTCC497 | EUG/AZI (µg/mL) | |||
| EUG | AZI | |||
| Alone MIC | 200 | 512 | ||
| Combination MIC | 12.5 | 64 | ||
| FICI | 0.187 | |||
| Type of interaction | Synergy | |||
| 4. CAJ-01 plus S. mutans MTCC497 | EUG/FLZ (µg/mL) | EUG/AZI (µg/mL) | ||
| EUG | FLZ | EUG | AZI | |
| Alone MIC | 800 | 1024 | 800 | 1024 |
| Combination MIC | 25 | 128 | 12.5 | 128 |
| FICI | 0.156 | 0.140 | ||
| Type of interaction | Synergy | Synergy | ||
| 5. CAJ-12 plus S. mutans MTCC497 | EUG/FLZ (µg/mL) | EUG/AZI (µg/mL) | ||
| EUG | FLZ | EUG | AZI | |
| Alone MIC | 200 | 2048 | 200 | 1024 |
| Combination MIC | 12.5 | 256 | 3.12 | 128 |
| FICI | 0.187 | 0.140 | ||
| Type of interaction | Synergy | Synergy | ||
EUG eugenol, FLC fluconaole, AMB amphotericin B, AZI azithjromycin