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. 2020 Oct 6;11:550827. doi: 10.3389/fimmu.2020.550827

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Copyright © 2020 Deshmukh, Speth, Sheppard, Neurauter, Würzner, Lass-Flörl and Rambach.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time and dose curve of GAG-dependent complement deposition on platelets. (A) Platelets were incubated for 5–90 min with serum as complement source in the presence of either medium, 20% supernatant (SN) of AF293, or 20% SN of Δuge3. (B) Platelets were incubated for 90 min with serum as complement source in the presence of 2–50% SN of AF293 or Δuge3. GAG deposition and opsonization were evaluated by flow cytometry. Each experiment was repeated at least three times with different platelet donors and with triplicate samples. Results are analyzed in comparison with the medium sample, which was set to 100%. ***p < 0.005, **p < 0.01, *p < 0.05.