Table 2.
Experimental expression of single viral DNA-binding proteins in context of plasmid injections, promote production of anti-dsDNA antibodies and to a variety of chromatin proteins.
| Plasmid | promoter |
Expressed antigen |
Anti-T antigen antibodies |
Anti-dsDNA antibodies |
Anti-Histone antibodies | Anti-TBP* | Anti-CREB** | References |
|---|---|---|---|---|---|---|---|---|
| pRSV-BKT | RSV LTRt | Pyv T ag | 681 ± 40 *** | 715 ± 69 | 557 ± 112 | 318 ± 99 | 402 ± 91 | (50, 147) |
| pRcCMV-BLT | HCMV ie-1 | Pyv T ag | 1282 ± 186 | 871 ± 53 | 763 ± 87 | 468 ± 254 | 552 ±186 | (50, 147) |
| pBS-BLT | None | Not expressed | Not detected | Not detected | Not detected | Not detected | Not detected | (50, 147) |
| pRcCMV-SLT155 | HCM ie-1 | SV 40 T ag | 573 ± 34 | 360 ± 46 | 259 ± 52 | Not tested | Not tested | (50, 147) |
| pRcCMV-SLT155T>S | HCM-ie-1 | SV 40 T ag Mutant non-dsDNA binding |
442 ± 44 | 29% ± 2, 5**** | Not detected | Not tested | Not tested | (50, 147) |
| pRA 17 | SCM-ie 1 | EBNA 1 (69.7 kDa) | Not tested | EBNA-1, dsDNA, Sm | Not tested | Not tested | Not tested | (49) |
All reported experiments are performed in BALB/c mice.
TATA-binding protein.
cAMP-response element binding protein.
The results are given as titers. These were determined by ELISA analyses of the induced serum antibodies aimed to quantify autoantibodies in mice injected by various T antigen expressing/non-expressing plasmids. The titers were determined from 2-fold dilution curves starting from dilution 1/100. The titers were defined as the reciprocal value of the dilution giving 50% of maximal binding to wells, as determined by individual reference sera (147).
These sera gave only marginal binding values as their binding at 1/100 dilution gave only 29% of the binding of a reference serum included in these assays.