Fig. 3.
Impact of isolated fecal strains on the viability of Caco-2 cells and the production of IL-8. Caco-2 cells (1 × 105 cells/well) were seeded into 24-well plates, grown for 7 days and then incubated for 18 hr in a fresh medium containing isolated fecal microbes (1 × 107 CFU/mL for each strain). (A) Cell viability was examined by MTT assay. The supernatants were collected for the measurement of (B) IL-8 production by ELISA. Results are representative of three independent experiments, each in triplicate. Data are expressed as the mean ± SEM of triplicate experiments. *p<0.05 compared with the phosphate-buffered saline (PBS)-treated group.