Fig. 6.

Impact of isolated fecal strains on allergen-induced IL-17, IL-10 and TGF-β secretion by mesenteric lymph node (MLN) cells. Mice in the OVA-sensitized and challenged (VH) group were treated as described in the Materials and Methods. The mice were sacrificed after the last challenge, and their MLNs were harvested. The MLN cells (5 × 10⁶ cells/mL) were treated with each identified fecal strain (1 × 10⁷ CFU/mL) and re-stimulated with OVA (50 µg/mL). After culture for 72 hr, the levels of (A) IL-17, (B) IL-10 and (C) TGF-β in the supernatants were quantified by ELISA. Results are representative of three independent experiments, each in triplicate. Data are expressed as the mean ± SEM of triplicate experiments. ^#p<0.05 compared with the phosphate-buffered saline (PBS) treatment. *p<0.05 compared with the OVA re-stimulation.