Figure 3.

GzmB and IFN-γ producing T cells are increased in Mavs^−/− mice during primary RSV infection. Percentage and total number of granzyme B (GzmB) and/or IFN-γ producing CD8⁺ T cells in (A) lung and (B) airways on day 8 in RSV-infected wt and Mavs^−/− mice, was determined by flow cytometry. (C) GzmB and (D) IFN-γ levels in BAL fluid of RSV-infected and mock-infected (PBS) mice were determined by ELISA. Data are represented as mean ± SEM. Data in (A, B) are pooled from two independent experiments with four to five mice per group (n = 9–10). Statistical significances of differences between indicated genotypes at each time point was determined by unpaired Student’s t test. Data in (C, D) are pooled from three independent experiments with three to five mice per group (n = 14–15). Each symbol represents one individual mouse. Statistical significances of differences between groups were determined by one-way ANOVA with Tukey’s post hoc test. *P < 0.05; ***P < 0.001.