Figure 3.

The levels of TDP-43 and phosphorylated TDP-43 are not altered in BV-2 cells harboring the C9orf72 hexanucleotide repeat expansion. TDP-43 phosphorylation (p-TDP-43) at site S409/S410 was assessed 24 and 48 h after transfection of BV-2 cells with either 66R plasmid or control plasmid (2R). (A) Representative Western blot images of four independent experiments are shown. Quantification shows mean ± SD of p-TDP-43 levels normalized to total TDP-43 levels (B) and total TDP-43 levels normalized to those of β-actin (C) from four independent experiments each with two to three biological replicates. For each experiment and time point, 2R was set as 100%. Two-tailed independent samples t-test (B,C) was performed. n = 11, df = 20 (24 h); n = 12, df = 22 (48 h). df, degrees of freedom; kDa, kilodalton; TDP-43, TAR DNA-binding protein 43.