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. 2020 Oct 6;10:573501. doi: 10.3389/fonc.2020.573501

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Copyright © 2020 Liu, Du, Wang, Gao, Li, Li, Sun, Binang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Primary data collection and global screening of microRNA (miRNA) expression to predict lymph node (LN) metastasis. (A) The morphology of exosomes was identified using transmission electron microscopy (TEM). Scale bar, 100 nm. (B) Size distribution and concentration of exosomes. (C) Western blot assay of CD9 and TSG101 in exosome samples (E) and exosome-depleted supernatants (EDSs). (D) Volcano plot showing the differentially expressed miRNAs from next-generation sequencing (NGS) analysis. (E) Comparison of internal control exosomal miR-16 expression among HD (n = 38), reflux esophagitis (n = 30), Barrett's esophagus (n = 18), esophageal leiomyoma (n = 15), LN– (n = 30), and LN+ (n = 30) detected by qRT-PCR.