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. 2020 Oct 6;11:573723. doi: 10.3389/fphys.2020.573723

FIGURE 3.

FIGURE 3

Knockdown of Dstac in body wall muscles reduced larval locomotion. (A) DstacRNAi larvae in which Dstac was knocked down in body wall muscles (Mef2:GAL4 > UAS:DstacRNAi) showed decreased locomotion compared with control LuciferaseRNAi larvae (Mef2:GAL4 > UAS: LuciferaseRNAi). Control n = 59, DstacRNAi n = 56. One-tailed, unpaired t test. (B) Labeling T tubules of body-wall muscles with anti-DLG showed that T tubules of DstacRNAi larvae (Mef2:GAL4 > UAS:DstacRNAi) are similar in morphology and structure to control LuciferaseRNAi larvae (Mef2:GAL4 > UAS:LuciferaseRNAi). The images are a single focal plane. Scale bar, 3 μm. (C) DstacRNAi larvae in which Dstac was knocked down in body wall muscles (Mef2:GAL4 > UAS:DstacRNAi) could develop to mature pharate adults in the pupae but couldn’t eclose. The left lower image shows a control LuciferaseRNAi pupa and a DstacRNAi pupa that are similar in morphology. The right lower image shows a pharate adult of DstacRNAi that was dissected out from its cocoon in which the wings are not inflated and did not survive. Scale bar, 1 mm. ****P < 0.0001.