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. 2020 Oct 6;14:556700. doi: 10.3389/fnins.2020.556700

FIGURE 5.

FIGURE 5

Effect on cells in the inner nuclear layer. (A) Amacrine cells were labeled with calretinin (green) and bipolar cells with protein kinase C alpha (PKCα) (red). Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). (B) No differences were observed regarding the number of calretinin+ cells between all groups at 4 and 8 days. (C) An upregulation of the relative CALRETININ mRNA expression was detected in filter retinas compared to control samples at 4 days (p = 0.001). No differences were noted at 8 days. (D) The number of bipolar cells was not altered in any group at 4 days. However, fewer PKCα+ cells were discovered in control (p = 0.04), filter (p = 0.02), and tweezers retinas (p = 0.02) compared to those in native samples at 8 days. (E) A significant PKCα mRNA downregulation was observed in filter samples compared to control ones at 8 days (p = 0.02). ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer. Scale bar: 20 μm, values are mean ± SEM for immunofluorescence (IF) and median ± quartile + min/max for quantitative real-time PCR (RT-qPCR). IF: n = 9–10/group; RT-qPCR: n = 5/group. *p < 0.05 vs. native group; #p < 0.05 and ##p < 0.01 vs. controls.