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. 2020 Oct 6;7:586146. doi: 10.3389/fmolb.2020.586146

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Copyright © 2020 Jovanović, Dragoj, Zhukovsky, Dar’in, Krasavin, Pešić and Podolski-Renić.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The combined effect of 5 or 6 with TMZ in glioma cell lines. Cell growth inhibition by TMZ in single and combined treatments with 5 and 6 in C6, RC6, U87, and U87-TxR cells determined by MTT assay. Mean values ± SEM were obtained from three independent experiments (n = 3). Statistical significance to TMZ alone: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), and p < 0.0001 (****). The interactions between 5 or 6 and TMZ was analyzed by CalcuSyn software. CI < 1 indicates synergistic effect, while CI close to 1 point to additive effect.