Figure 1.

TGFβ receptor activates PDGFRβ. A, TGFβ increases activating phosphorylation of PDGFRβ at Tyr-857. Mesangial cells were incubated with 2 ng/ml TGFβ for the indicated duration. The cleared cell lysates were immunoblotted with phospho-PDGFRβ (Tyr-857) and PDGFRβ antibodies. B–J, mesangial cells were treated with 5 µm SB (panels B–D) or 0.1 µm JNJ (panels E–G) or transfected with siRNAs against PDGFRβ or scrambled RNA (panels H–J) prior to incubation with 2 ng/ml TGFβ for 2 h. The cell lysates were immunoblotted against the indicated antibodies. Molecular weight markers are shown in the left margins.