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TGFβ increases PDGF B expression to augment activating phosphorylation of PDGFRβ and Akt. A, mesangial cells were incubated with TGFβ (2 ng/ml) for the indicated period of time. B and C, mesangial cells were transfected with siPDGF B or scrambled RNA prior to incubation with TGFβ (2 ng/ml) for 2 h. The cell lysates were immunoblotted with the indicated antibodies.
