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PDGF increases expression of TGFβ to induce its signaling. A, mesangial cells were incubated with PDGF (20 ng/ml) for the indicated period of time. B–F, mesangial cells were treated with 0.1 µm JNJ (panel B) or 5 µm SB (panels C and D) or 1 μg/ml IgG or TGFβ antibody (panels E and F) prior to incubation with PDGF for 2 h. The cell lysates were immunoblotted with the indicated antibodies.
