Steady-state ATPase activity of IFI, EMB, and exon 3 chimeric S1 isoforms.
A–D, basal Ca-ATPase activity (A), basal Mg-ATPase (B), actin-activated Mg-ATPase activity (Vmax) (C), and the turnover number (kcat) for acto-S1 (D) were determined as described under “Experimental Procedures.” Notations above histograms indicate the level of statistically significant differences (*, p < 0.05; ****, p < 0.0001; ns, not statistically significant). Significant differences were assumed for p < 0.05.