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. 2020 Aug 5;295(42):14279–14290. doi: 10.1074/jbc.RA120.013905

Figure 1.

Figure 1.

YFP-tagged BBSome subunits localize to the primary cilia and rescue the BBSome deficient cell lines. A, YFP-tagged BBSome subunits localize to the ciliary base and primary cilia upon serum starvation in WT RPE1 cells. Antibody against Ac-tub was used to stain the primary cilia. The nucleus was stained with DAPI. Scale bar, 5 μm. B, plots show the comparison of ciliary length between WT, BBS KO, and reconstituted BBS KO RPE1 cell lines. Ciliary length was rescued upon expression of the respective YFP-tagged BBSome subunit. Cilia length measurements were carried out using the Fiji ImageJ software. Medians with interquartile range from three independent experiments of n > 100 are shown. Statistical significance was calculated using two-tailed Mann–Whitney test. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. C, plot shows the comparison of ciliary length between WT, BBS8 KO, and reconstituted BBS8 KO RPE1 cell lines. Ciliary length was restored to WT length upon expression of YFP-BBS8 in BBS8 KO cell line. Cilia length measurements were carried out using the Fiji ImageJ software. Medians with interquartile range (error bars) from three independent experiments of n > 100 are shown. Statistical significance was calculated using two-tailed Mann–Whitney test. ***, p < 0.001.