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. 2020 Oct 6;11:569104. doi: 10.3389/fimmu.2020.569104

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Copyright © 2020 Zhang, Liu, Liu, Zhong, Li, Ran, Guo, Chen, Zhao, Wang and Zhou

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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B420 inhibited the RIP3 signaling pathway of liver macrophages in EAH mice (A) Representative fluorescence images of liver tissues co-stained with F4/80 and RIP3. F4/80 (green), RIP3(red), DAPI (blue). (B) Representative fluorescence images of liver tissues co-stained with F4/80 and MLKL. F4/80 (green), MLKL (red), DAPI (blue). (C) Total RNA was extracted from the liver tissues for real-time PCR analysis. The relative expressions of RIP3 and MLKL were shown. (D) Protein levels of RIP3 and MLKL in the liver tissues from each group were detected by western blotting and the relative intensity was measured. In (A–D), n=6 in each group. Scale bar: 50μm. The data were presented as means ± SD (Student’s t-test, *p < 0.05, **p < 0.01).