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. 2020 Oct 6;11:569104. doi: 10.3389/fimmu.2020.569104

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Copyright © 2020 Zhang, Liu, Liu, Zhong, Li, Ran, Guo, Chen, Zhao, Wang and Zhou

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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B420 regulated pro-inflammation cytokines and chemokines in liver as well as Th17 cells in liver and spleen. Total RNA was extracted from the liver tissues for real-time PCR analysis and mononuclear lymphocytes were isolated from liver and spleen, counted and stained with cell markers to identify Th17 cells. (A) The relative expressions of TNF-α, IL-6 and IL-1β in liver tissues were analyzed by real-time PCR. (B) The relative expressions of CCL2 and CCR2 mRNA in liver tissues were detected. (C) Typical CD4+IL-17+ Th17 cells flow cytometric plots and the percentage of Th17 cells out of the liver CD4+population were calculated. (D) Representative flow cytometry plots and the percentage of Th17 cells out of the spleen CD4+population were calculated. In (A–D), n=6 in each group. The data were presented as means ± SD (Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001).