Table 4.
The Observed Frequency of BCR-ABL1 TKD Mutations in Different Studies
| Study | Method of the detection | Overall frequency of BCR-ABL1 mutations (%) |
Common hotspot BCR-ABL1 TKD mutations (%) | CML phases |
|---|---|---|---|---|
| This study (Thai study) |
Direct sequencing | 20 (49/245) | Single mutation (13) Q252H (0.4) F317L (1) Y253H (0.4) A350T (0.4) E255K (2) F359I/V (1) D276G (0.4) E455K (0.4) F311I (0.4) E459K (0.4) T315I (7) F486S (0.4) Compound/polyclonal mutations (7) |
NA |
| Elias et al., (2014 )(Malaysian study) (Elias et al., 2014) |
D-HPLC and Direct sequencing | 22 (28/125) | Single mutation G250E (2) M351T (2) G251E (1) E355A/G (2) Y253H (1) F359C (2) E255K (4) N368S (1) D276G (1 L387M (1) V289F (1) H396R (1) T315I (7) A397P (1) Compound/polyclonal mutation (NA) |
CP,AP,BP |
| Meggyesi et al., (2012) | Direct sequencing | 20 (15/74) | Single mutation | CP,AP,BP, Ph positive ALL |
| (Hungarian study) (Meggyesi et al., 2012) |
M244K/V (8) M351T (5) G250E (1) E355G (1) Y253H (1) F359I/V (3) E255V (4) L384M (1) D276G (1) L387M (1) E279K (1) H396R (1) T315I (5) Compound/polyclonal mutation (NA) |
|||
| Kim et al., (2009) (Korean syudy) (Kim et al., 2009) |
Direct sequencing and ASO-PCR | 51 (70/137) | Single mutation M244V (9) E355G (1) G250E (10) F359C (1) Q252H (4) F359I/V (5) Y253F/H (14) H396P/R (2) E255K/V (17) S417Y (1) T315I (23) E450K (1) F317L (4) E459K (4) M351T (2) P480L (1) Compound/polyclonal mutation (NA) |
CP,AP,BP |
| Branford et al., (2003) (Australian study) (Branford et al., 2003) |
Direct sequencing | 19 (27/144) | Single mutation (14) M244V (1) M351T (6) L248V (1) E355G (2) G250E (1) F359V (1) Q252H (3) H396R (1) Y253F (1) S417Y (1) E255K/V (4) E459K (1) T315I (1) F486S (1) F317L (1) Compound/polyclonal mutations (5) |
CP,AP |