FIG 1.

Sequence analysis of the Tat loci in B. melitensis M28. (A) Genetic organization of the Tat loci in B.melitensis M28. tatABC in M28 is found on chromosome I and has been designated BM28_A0889, BM28_A0890, and BM28_A0891. Similar to B. abortus 2308, M28 tatABC are flanked by ScpB (encoding an SMC-Scp complex subunit) and serS (encoding a serine-tRNA ligase). Genes represented by arrows are drawn to scale. (B) Sequence alignment of TatA from M28 and other bacteria, as well as TatB of M28, illustrates the conservation of a transmembrane segment (TMS) and an amphiphilic helix (APH). The graph was created by ClustalW. Color scheme illustrates the different types of amino acids: blue, hydrophobic; red, positively charged; magenta, negatively charged; green, polar; orange, glycine; pink, cysteine; yellow, proline; cyan, aromatic; white, unconserved.