TABLE 1.

Tat substrates identified by bioinformatics and experimental approaches in B. melitensis M28

Gene ID	Predicted gene product	N-terminal signal sequence	Verified by amidase reporter assay
Substrate/solute-binding protein
A0009	ABC transporter substrate-binding protein	MTFLCMNRRHFMGLSGSAALVACLPGQAFA	+
A0376	TRAP transporter substrate-binding protein	MKETLSRRSFLTKGAAIGAAAATSGAALATPAIA	+
B0279	ABC transporter substrate-binding protein	MTTTRRDILLGAAAFGLASIATRLPAYA	+
B0446	ABC transporter substrate-binding protein	MHENNEIGQLDETGAALAEENTHMKFSLNRRQALLGMGAAGAAVAFGFPARA	+
B0520	Periplasmic substrate-binding protein	MNIANIFDRMNFRRAVMFTVAAIGLTASPLFTAPAQA	+
B0754	ABC transporter substrate-binding protein	MSGFVASRRAFLVGSTSLAALAFWPKATWA	+
B0779	ABC transporter substrate-binding protein	MRLTRRQALGGMAALAAFPASRVWA	+
B0934	MetQ/NlpA family ABC transporter substrate-binding protein	MSSVLSRYALTRRAGLKALLFTAAALTVGFASAPSHA	+
B1133	D-xylose ABC transporter substrate-binding protein	MKRRNFLAGALVAAALGIGAMGATPAFA	_
Oxidoreductases
A0396	Disulfide bond reductase DsbA family protein	MMNRRQILAATAAGAVKFALSGGSGLA	+
A0515	DsbA family protein	MPVALNRRHVISLAGAAAAGLAFTRGANA	_
A1551	Ubiquinol-cytochrome c reductase iron-sulfur subunit	MSAHDTAEPTRRDFLYIATGMAGVVGVGG	_
		LAWPFIDQMRPDASTLAAA
A1757	Thiamine/thiamine pyrophosphate ABC transporter permease ThiP	MTATPARRTSLASPATKPVAGGLALAFLATL	_
		AGGALLALA
B0019	Methionine sulfoxide reductase catalytic subunit YedY	MTGPRLSRRRFLTFTGMAGSAVLLSGCDA	+
B0251	Nitrite reductase, copper-containing	TAT-P: MADQIQVNRRTILAGAALAGALGPVLSATSAWG	+
		PRED-TAT: MADQIQVNRRTILAGAALAGALGPVLSATSAWGQGTMRKASA	+
B0672	Multicopper oxidase CueO	MTGITRRRLLALGASAACVAALRPLGAFA	+
B0965	Methionine sulfoxide reductase catalytic subunit MsrP	MSSFKPSRFSTARLTGDAVTPKSIYLRRREFMIGLGAIATTGAASSAFA	+
Cell envelope biosynthesis
A0577	L,d-transpeptidase	PRED-TAT: MQTTLTRRSFLTAMTATAATGLAGCAQLG	+
		TAT-P: MQTTLTRRSFLTAMTATA	_
A0773	Murein L,d-transpeptidase	MTKTDRPANAFRADRRRFLRSAATAGLSVAVSAMVSSAYA	+
A1479	Linear amide C-N hydrolase	METKSSLWKSSRRVLAHGAATVLVAAGLIVPQAAMA	+
B0388	O-antigen/exopolysaccharide biosynthesis protein	MIISDVSSANGRQPVADLLETIKRRKLMLTLPIIAGVGVGFAGYLTAPVSY	+
Others
A0340	CAP domain-containing protein	MQQKHGIRLSRRGFLMLAGGAMALSALPVDWAQA	+
A1724	5-Formyltetrahydrofolate cyclo-ligase	METAGQGRSDEKQALRRVVLA	_
B0340	P1 family peptidase	MDRRTFAKSLAGLGAMPFAVNTASAGA	+
B0737	Hint domain-containing protein	MSSELNGHSPRNRARRHFLGVAAAAAARVAILGALVSSSLPARA	+
RS03245	TIGR02301 family protein	MKDYRRLFRLAAGAAALWLAVCPPVLA	+
B0249	Lipase	MNRRSLLLGLLAGAALVLPSLPAEA	+
B0604	Amidohydrolase	MCLMCGPAAKTILNITRRNLLKGLGAASLTAAIPFRAES	+