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. 2020 Oct 19;88(11):e00293-20. doi: 10.1128/IAI.00293-20

FIG 6.

FIG 6

Interaction between primary human neutrophils and a SAC. (A) Numbers of S. aureus JAR 06.01.31 CFU cultured at 6 or 24 h after the addition of primary human neutrophils to mature in vitro SACs that were nontreated or plasmin pretreated. Holm-Sidak’s multiple-comparison test was used. Data are mean ± SD numbers of CFU per sample and are from three independent experiments. (B and C) Confocal images of a mature in vitro SAC exposed for 6 (B) or 24 (C) h to primary human neutrophils. (D and E) Invasion of a plasmin-pretreated mature SAC by primary human neutrophils after 6 (D) or 24 (E) h of exposure. These SACs were treated with plasmin for 2 h and subsequently exposed for 6 or 24 h to primary human neutrophils. Samples for confocal imaging were stained with propidium iodide (PI) and Syto9. Green, both bacteria and primary human neutrophils stained with nucleic acid dye Syto9, indicating all cells. Red, PI-stained cells, indicating dead cells. Scale bars, 100 μm (B and C), 50 μm (D), and 100 μm (E). N.S., P > 0.05; *, P < 0.05.