TABLE 2.
Isotypes, epitope features, domain specificities, and epitope residues of antiadhesin MAbs
| MAb | Isotype | Epitope feature(s) | Domain specificitya | Epitope residue(s) |
|---|---|---|---|---|
| Anti-CfaE MAbs | ||||
| P8D10 | IgG1 | Conformational | AD | R67, R181 |
| P6C11 | IgG2b | Conformational | AD | R67, R181 |
| P6H4 | IgG1 | Conformational | AD | R67, R181 |
| P10A7 | IgG1 | Conformational | AD | R67, S86b , R181 |
| P5C7 | IgG1 | Conformational | AD | R67, S86b , R181 |
| P2E11 | IgG1 | Conformational | PD | NDc |
| P3B2 | IgG1 | Conformational | PD | ND |
| P13A7 | IgG1 | Linear | PD | ND |
| P1F9 | IgG1 | Conformational | PD | ND |
| Anti-CsbD MAbs | ||||
| P7C2 | IgG1 | Conformational | AD | ND |
| P9A5 | ND | Conformational | AD | ND |
| P2H6 | IgG1 | Conformational | AD | T84 |
| P6G1 | IgG1 | Conformational | AD | ND |
| P2A9 | IgG1 | Conformational | AD | ND |
| P1F7 | IgG1 | Conformational | AD | H144 |
| P9E11 | ND | Conformational | AD | ND |
| P7F9 | IgG1 | Linear/conformational | AD | S88, R181, Y182 |
| P5A12 | IgG1 | Conformational | AD | ND |
| P9D12 | ND | Linear | PD | ND |
| P7F12 | IgG1 | Linear | PD | ND |
| Anti-CotD MAbs | ||||
| P7F6 | IgG1 | Conformational | AD | ND |
| P3F4 | IgG2a | Conformational | AD | ND |
| P6B8 | IgG1 | Conformational | AD | R69, R184 |
| P3D11 | IgG1 | Conformational | AD | ND |
| P9A10 | IgG1 | Linear | AD | ND |
| P9G7 | IgG1 | Linear | PD | ND |
| P2B8 | IgG1 | Linear | PD | ND |
| P12A2 | IgG1 | Linear | PD | ND |
a
AD, adhesin domain; PD, pilin domain.
b
The epitope residue was identified by hemagglutination inhibition assay.
c
ND, not determined by the methods used in this study.