TABLE 3.
Functional activities of antiadhesin MAbs measured by the minimum concentrations to inhibit hemagglutination induced by eight ETEC strains expressing class 5 fimbriae
| MAb | MIC (μg/ml) against MRHA caused by ETEC strain (in parentheses) expressing indicated fimbriae of subclassa
: |
|||||||
|---|---|---|---|---|---|---|---|---|
| 5a |
5b |
5c |
||||||
| CFA/I (H10407) | CS4 (BANG10-SP) | CS14 (WS3294A) | CS17 (WS6788A) | CS17 (LSN02-013966/a)b | CS17 (WS4240A)c | CS1 (WS1974A) | CS2 (C91f) | |
| Anti-CfaE MAbs | ||||||||
| P8D10d | 1.2 | — | — | — | — | ND | — | — |
| P6C11d | 1.2 | — | — | — | — | ND | — | — |
| P6H4d | 1.6 | — | — | — | — | ND | — | — |
| P10A7d | 0.8 | — | — | 8.0 | 187.5 | — | — | — |
| P5C7d | 1.2 | — | — | 16.0 | — | — | — | — |
| P2E11 | 31 | — | — | — | — | ND | — | — |
| P3B2 | 3 | — | — | 12.0 | 31.3 | ND | 188 | — |
| P13A7 | >400 | >400 | >400 | 13.0 | >400 | ND | >400 | >400 |
| P1F9 | 1.5 | — | — | — | — | ND | — | — |
| Anti-CsbD MAbs | ||||||||
| P7C2d | — | — | — | 1.0 | 1.0 | 2.0 | 4 | — |
| P9A5d | — | — | — | 4.0 | 3.9 | ND | 4 | — |
| P2H6d | — | — | — | 4.0 | 31.3 | 375 | 31 | — |
| P6G1d | — | — | — | 8.0 | 15.6 | ND | 16 | — |
| P2A9d | — | — | — | 8.0 | 15.6 | ND | 16 | — |
| P1F7d | — | — | — | 0.3 | — | ND | — | — |
| P9E11d | — | — | — | 4.0 | — | ND | — | — |
| P7F9d | — | — | — | — | — | ND | — | — |
| P5A12d | — | — | — | — | — | — | — | — |
| P9D12 | — | — | — | 63 | — | ND | — | — |
| P7F12 | — | — | — | — | — | ND | — | — |
| Anti-CotD MAbs | ||||||||
| P7F6d | — | — | — | — | — | ND | — | 0.5 |
| P3F4d | — | — | — | — | 250 | ND | — | 1.0 |
| P6B8d | — | — | — | — | — | ND | — | 3.0 |
| P3D11d | — | — | — | — | — | ND | — | 16.0 |
| P9A10d | — | — | — | — | — | ND | — | 188 |
| P9G7 | — | — | — | — | — | ND | — | — |
| P2B8 | — | — | — | — | 250 | ND | — | 250 |
| P12A2 | — | — | — | — | — | ND | — | 125 |
a
MAbs with MICs of ≤10 μg/ml, 10 to 100 μg/ml, and 100 to 250 μg/ml were defined in this study as having strong, moderate, and low functional activity, respectively. —, the MIC was greater than 250 μg/ml; ND, not determined (the experiments were not performed).
b
The CS17+ LSN02-013966/A strain contains the CsbD/L85I/H144A allelic variation from the sequence of the CS17+ WS6788A strain.
c
The CS17+ WS4240A strain contains the CsbD/L85I allelic variation from the sequence of the CS17+ WS6788A strain.
d
The MAb was adhesin domain specific.