FIG 4.

Rapamycin treatment of E. faecalis-induced DCs upregulated the expression of costimulatory molecules. (A) Scattergrams from flow cytometry of percentage expression of MHCII, CD86, and CD80 by E. faecalis-induced CD11c⁺ DCs that were treated with mTORC1 inhibitor rapamycin relative to that in the control groups shown. Upper right quadrants show percent double-positive CD11c⁺ MHCII⁺ (top), CD11c⁺ CD86⁺ (middle), and CD11c⁺ CD80⁺ (bottom) DCs. (B) A bar graph showing the mean percentages of double-positive CD11c⁺ MHCII⁺, CD11c⁺ CD86⁺, and CD11c⁺ CD80⁺ cells in the four groups. The experiment was repeated in triplicates three times.