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. 2020 Oct 19;88(11):e00252-20. doi: 10.1128/IAI.00252-20

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FIG 3 — Serum and fecal antibody responses in mice immunized i.n. or i.d. with chimera. Mice (10/group) were immunized i.d. on days 0, 14, and 28 with 21 μg chimera, 8 μg dsc₁₉CfaE with or without 0.1 μg LT(R192G), 0.1 μg LT(R192G), or PBS. Three additional groups of mice were immunized i.n. with 21 μg chimera, 8 μg dsc₁₉CfaE plus 0.1 μg LT(R192G), or PBS. (A) Serum anti-CfaE IgG antibody response on day 42; (B) serum anti-LTB IgG antibody response on day 42; (C) fecal anti-CfaE IgA antibody response on day 33; (D) fecal anti-LTB IgA antibody response on day 33. ELISA results (panels A to D) are presented as mean log₁₀ titers ± SD. (E) Functional antibody titers measured by the HAI assay (median ± interquartile ranges). Horizontal dotted lines denote the limit of detection for the assays. Statistical significance was determined by using a one-way ANOVA and Tukey’s post hoc test. ns, not significant.