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. 2020 Oct 19;88(11):e00252-20. doi: 10.1128/IAI.00252-20

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FIG 4 — Serological immune responses in guinea pigs immunized i.d. with chimera. Animals (24/group) were immunized i.d. on days 0, 21, 42, and 63 with 13 μg chimera with or without 0.1 μg LT(R192G), 25 μg dsc₁₉CfaE with or without 0.1 μg LT(R192G), or PBS. (A) Anti-CfaE IgG antibody responses (mean ± SD); (B) functional antibody titers measured by the HAI assay (median ± interquartile ranges); (C) anti-LTB IgG antibody response (mean ± SD). Horizontal dotted lines denote the limit of detection of the assays. Statistical significance was determined by using one-way ANOVA and Tukey’s post hoc test.